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ABSTRACT Fungal contamination of drinking water distribution systems can impact water quality with implications for public health. We document an instance of Exophiala spp. biofilm contamination of customer taps in the Midwest United States following consumer complaints. Three samples of black biofilm were collected from customer taps in Ohio and then processed using next-generation DNA sequencing of the bacterial 16S and fungal ITS regions. Two samples with successful ITS sequencing were dominated by Exophiala spp., putatively identified as E. cancerae and E. lecanii-corni. Dominant bacterial phyla in samples included Proteobacteria, Bacteroidetes, Actinobacteria, and Acidobacteria. Bacterial composition varied substantially at the family and genus levels, and potentially pathogenic bacteria (i.e., Acinetobacter spp., Legionella spp., Mycobacterium spp., and Pseudomonas spp.) were detected. The potential for fungal contamination of drinking water distribution systems should be evaluated when biofilms are observed. 

The COVID-19 pandemic exposed the limits of big data to guide decision-making in times of crisis. As people navigated daily life, they were confronted with the reality that data were often not yet material but rather in-the-making. Drawing upon critical and feminist lenses and participatory methodologies, this study investigates the data stories of nine people of Asian descent living in the United States. Findings illustrate how participants navigated within and across time, space, activity, media, epistemology, race, and politics to produce lively data assemblages. These data stories guided social-distancing and mask-wearing weeks before official US policy even as participants lived in constant fear of dehumanizing racist and xenophobic violence. This study advances theorizing about data practices for human knowing and learning with media, racial and epistemic (in)justice, and community action. It also advances participatory research as a site of epistemic resistance and activism. 

The plant pathogenPseudomonas syringaesecretes multiple effectors that modulate plant defenses. Some effectors trigger defenses due to specific recognition by plant immune complexes, whereas others can suppress the resulting immune responses. The HopZ3 effector ofP.syringaepv. syringae B728a (PsyB728a) is an acetyltransferase that modifies not only components of plant immune complexes, but also thePsyeffectors that activate these complexes. In Arabidopsis, HopZ3 acetylates the host RPM1 complex and thePsyeffectors AvrRpm1 and AvrB3. This study focuses on the role of HopZ3 during tomato infection. InPsy-resistant tomato, the main immune complex includes PRF and PTO, a RIPK-family kinase that recognizes the AvrPto effector. HopZ3 acts as a virulence factor on tomato by suppressing AvrPto1_Psy-triggered immunity. HopZ3 acetylates AvrPto1_Psyand the host proteins PTO, SlRIPK and SlRIN4s. Biochemical reconstruction and site-directed mutagenesis experiments suggest that acetylation acts in multiple ways to suppress immune signaling in tomato. First, acetylation disrupts the critical AvrPto1_Psy-PTO interaction needed to initiate the immune response. Unmodified residues at the binding interface of both proteins and at other residues needed for binding are acetylated. Second, acetylation occurs at residues important for AvrPto1_Psyfunction but not for binding to PTO. Finally, acetylation reduces specific phosphorylations needed for promoting the immune-inducing activity of HopZ3’s targets such as AvrPto1_Psyand PTO. In some cases, acetylation competes with phosphorylation. HopZ3-mediated acetylation suppresses the kinase activity of SlRIPK and the phosphorylation of its SlRIN4 substrate previously implicated in PTO-signaling. Thus, HopZ3 disrupts the functions of multiple immune components and the effectors that trigger them, leading to increased susceptibility to infection. Finally, mass spectrometry used to map specific acetylated residues confirmed HopZ3’s unusual capacity to modify histidine in addition to serine, threonine and lysine residues.